Characterize translation machinery alterations in thousands of tumors of diverse origins

Recurrent cancer mutations that disrupt protein-coding regions have been identified through large-scale projects employing targeted sequencing strategies such as whole exome sequencing. While our previous work demonstrated that exome sequencing can reveal critical noncoding mutations in cancer, much of the genome remains inaccessible to analyses when targeted sequencing approaches are... [Read More]

Develop a platform for discovery of trans-acting translation regulators

Regulatory proteins control translation by scaling global protein production and by modulating translation from specific mRNAs. Targeted approaches continue to reveal new translation regulatory proteins, however there is currently no genome-wide functional genomics approach to identify translation control factors. Our laboratory will address this fundamental gap by devising an assay... [Read More]

Determine cis-regulatory translation control elements in the human genome

Advances in sequencing have greatly accelerated our ability to catalog human genetic diversity, yet the functional consequences of these genomic differences are still poorly understood. Genetic variants typically exert phenotypic effects through impacts on protein function and abundance. By integrating RNA-seq data with transcription factor binding and chromatin profiles, significant... [Read More]